Quantitative Proteomics: Berndt Thiede's group

Thanks to the group for teaching me their isobaric labelling technique for quantitative proteomics this week. Will be great to use it for our cancer stem cell research as it is quick and relatively cheap.  It will also be nice to check out their software package, IsobariQ.

Interview on Norwegian radio on doping analysis

Here is a link to an interview with me this morning on Norwegian radio on doping analysis.

In addition I would like to recommend Prof. Peter Hemmersbach's paper "History of Mass Spectrometry at the Olymic Games".

Waters company Innovation prize

Congrats to Hanne Røberg Larsen for her award-winning presentation at the Hafjell mass spectrometry meeting!
The subject was a novel liquid chromatography column switching system that she uses for analysis of Hedgehog active oxysterols. She took home 150, 000 NOK (about 25,000 American Dollars) in Waters equipment.
We are currently typing up manuscripts describing the instrumentation and application.
Co-authors from our group are Kristin Opsal, Steven Ray Wilson, Siri Altern Pedersen, Ida Brenna.


I am currently working on a scheme to clone these excellent researchers.

HILIC and temperature: explanation of inverse Van´t Hoff plots

Hydrophilic Interaction Chromatography (HILIC) of nucleoside triphosphates with temperature as a separation parameter

Elin Johnsen, Steven Ray Wilson, Ingvild Odsbu, Andreas Krapp, Helle Malerod, Kirsten Skarstad and Elsa Lundanes
Journal of Chromatography A, (ahead of print):
doi:10.1016/j.chroma.2011.01.066

Eight deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs): ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP, were separated with two 15 cm ZIC-pHILIC columns coupled in series, using LC-UV instrumentation. The polymer-based ZIC-pHILIC column gave significantly better separations and peak shape than a silica-based ZIC-HILIC column. Better separations were obtained with isocratic elution as compared to gradient elution. The temperature markedly affected the selectivity and could be used to fine tune separation. The analysis time was also affected by temperature, as lower temperatures surprisingly reduced the retention of the nucleotides. dNTP/NTP standards could be separated in 35 minutes with a flow rate of 200 μL/minute. In Escherichia coli cell culture samples dNTP/NTPs could be selectively separated in 70 minutes using a flow rate of 100 μL/minute.

Cancer Research; 71(1); 197–205. 2010

Novel Synthetic Antagonists of Canonical Wnt Signaling Inhibit Colorectal Cancer Cell Growth 

Jo Waaler, Ondrej Machon, Jens Peter von Kries, Steven Ray Wilson, Elsa Lundenes, Doris Wedlich, Dietmar Gradl, Jan Erik Paulsen, Olga Machonova, Jennifer L. Dembinski, Huyen Dinh, Stefan Krauss

Canonical Wnt signaling is deregulated in several types of human cancer where it plays a central role in tumor cell growth and progression. Here we report the identification of 2 new small molecules that specifically inhibit canonical Wnt pathway at the level of the destruction complex. Specificity was verified in various cellular reporter systems, a Xenopus double-axis formation assay and a gene expression profile analysis. In human colorectal cancer (CRC) cells, the new compounds JW67 and JW74 rapidly reduced active β-catenin with a subsequent downregulation of Wnt target genes, including AXIN2, SP5, and NKD1. Notably, AXIN2 protein levels were strongly increased after compound exposure. Long-term treatment with JW74 inhibited the growth of tumor cells in both a mouse xenograft model of CRC and in Apc^Min mice (multiple intestinal neoplasia, Min). Our findings rationalize further preclinical and clinical evaluation of these new compounds as novel modalities for cancer treatment.

http://cancerres.aacrjournals.org/content/71/1/197.abstract